|B. Common histologic stains|
|H&E||Hematoxylin and eosin. Cytoplasm is pink and nuclei are blue. Used routinely for light microscopy.|
|GMS||Gomorri Methenamine Silver. Stains fungi including pneumocystis, black. Counterstain is green.|
|AFB||Acid Fast Bacilli (Ziehl-Neelson) AFB are stained pink/red. Counterstain is blue.|
|Fite||Modified AFB stain. Acid is dilute so weakly acid fast organisms eg atypical mycobacteria can be stained - colors are same as in AFB stain.
|Auramine-Rhodamine||Fluorescent dye visualized under ultraviolet light. Rapid screening, usually used for smears.|
|EVG||Elastic Van Gieson. Stains elastic tissue black. Counterstain varies from yellow to pink.|
|Trichrome||Masson trichrome most commonly used. Stains collagen (fibrous tissue) blue and muscle red.|
|Immunoperoxidase||"Brown stain." Section of tissue on glass slide is layered with primary antibody which may be monoclonal or polyclonal. Antigen-antibody reaction is then detected by peroxidase-labeled antibody. The color reaction is brown. Occasionally a red color reaction is used. Counterstain is hematoxylin (blue). The specificity lies not in the color but in the primary antibody used.